Welcome to the research lab of Shelly Sakiyama-Elbert in the Department of Biomedical Engineering at The University of Texas at Austin, Texas.
Our research program employs tissue and cellular engineering approaches to investigate biomedical problems. In particular, our lab is interested in the related areas of gene therapy, drug delivery, and stem cell biology with applications to therapies for spinal cord and peripheral nerve injury.
Shelly Sakiyama-Elbert, PhDDepartment Chair, Professor, Biomedical Engineering Ph.D. Chemical Engineering, M.S. Chemical Engineering, B.S. Chemical Engineering and Biology, |
Mary Alice SalazarLab Manager, 2017-2022 M.S. Chemistry COMSET Graduate Fellow Clemson UniversityB.S. Biochemistry, B.A. Biology Research Areas: Scaffold for nerve regeneration, growth factor delivery, spinal cord and peripheral nerve injury, microspheres |
Jaewon LeePh.D, Candidate, 2016--
B.S. Chemical Engineering, Research Areas: Microfluidics, biomimetics |
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Sangamithra VardhanPh.D, Candidate, 2017--
B.S. Biological Engineering, Research Areas: Stem cell engineering, astrocyte, hydrogels |
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Hayley LindsayPh.D, Candidate, 2018--
B.S. Biomedical Engineering, Research Areas: Stem cell engineering, corticospinal neurons, biomaterials |
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Pablo Ramos FerrerPh.D, Candidate, 2018--
B.S. Chemical Engineering, Research Areas: Biomaterials, growth factor delivery, drug delivery, spinal cord regeneration, hydrogels |
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Tyler N JordanPh.D, Candidate, 2020--
B.S. Chemical Engineering, Research Areas: Stem cell engineering, astrocyte, extracellular vesicles |
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Aditi MerchantBiomedical Engineering, Class of 2023 University of Texas at Austin Research Areas: Stem cell engineering, astrocyte, extracellular vessicles |
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Alaynah MurphyBiomedical Engineering, Class of 2022 University of Texas at Austin Research Areas: Biomaterials, growth factor delivery, drug delivery, spinal cord regeneration, hydrogels |
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Miriam GonzalezBiomedical Engineering, Class of 2022 University of Texas at Austin Research Areas: Stem cell engineering, differentiation, V1 interneurons |
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Morgan OwensBiomedical Engineering, Class of 2024 University of Texas at Austin Research Areas: Stem cell engineering, differentiation, V1 interneurons |
Nick WhitePh.D, Biomedical Engineering, University of Texas at Austin. 2022
B.S. Biomedical Engineering, Research Areas: Stem cell engineering, differentiation, V1 interneurons, Center pattern generator, 2015-2022 |
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Jennifer PardieckPh.D, Biomedical Engineering University of Texas at Austin. 2021
B.S. Biomedical Engineering, Research Areas: Stem cell engineering, differentiation, V0 interneurons, 2014-2021 |
Bill WangPh.D, Biomedical Engineering University of Texas at Austin. 2019
B.S. Bioengineering, Research Areas: Microfluidics devices, GDNF signalling 2014-2019 |
Russell ThompsonMD/Ph.D, Biomedical Engineering, 2014--
B.S. Chemistry Biology, Research Areas: Spinal cord injury, astrocytes, matrix scaffolding |
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Nisha IyerPh.D, Biomedical Engineering Washington University in St. Louis 2016 NRSA F31 Graduate Fellow
B.S. Biomedical Engineering, Research Areas: Stem cell engineering, V2a interneurons, microdevices, spinal cord injury 2012-2016 |
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Hao Xu, PhD
Ph.D, Biomedical Engineering
B.S. Biomedical Engineering, Research Areas: Stem cell engineering, V3 interneurons, differentiation 2010-2015 | |
Thomas Wilems, PhDPh.D, 2015 NSF Graduate Research Fellow
Ph.D. Biomedical Engineering,
B.S. Biomedical Engineering, Research Areas: Spinal cord injury, anti-inhibitory molecules, drug delivery 2011-2015, 2017-2018 |
Laura Marquardt, PhDPost-Doc, Stanford University NSF Graduate Research Fellow
Ph.D. Biomedical Engineering,
B.S. Biomedical Engineering, Research Areas: Schwann cells, peripheral nerve injury, acellular nerve grafts 2011-2014 |
Xi Lu, PhDPost-Doc, Uppsala, Sweden
Ph.D. Biomedical Engineering,
B.S. Biomedical Engineering, Research Areas: Parkinson's disease, microdevices 2009-2013 |
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Dylan McCreedy, PhDPost-Doc, Gladstone Institutes NSF Graduate Research Fellow
Ph.D. Biomedical Engineering,
B.S. Biomedical Engineering, Research Areas: Stem cell engineering, motor neurons, spinal cord injury, differentiation 2009-2013 |
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Nithya Jesuraj, PhDPost-Doc, Boston Scientific
Ph.D. Biomedical Engineering,
B.S. Chemical Engineering, Research Areas: Schwann cell phenotype, peripheral nerve injury 2008-2012 |
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Matthew WoodAssistant Professor, Department of Surgery
Ph.D. Biomedical Engineering, 2006-2009 |
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Philip JohnsonSenior Scientist, Holaira
Ph.D Biomedical Engineering, 2005-2009 |
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Stephanie WillerthAssistant Professor, Mechanical Engineering
Ph.D. Biomedical Engineering, 2004-2008 |
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Nicole MooreProgram Officer NCI/NIH, Physical Sciences
Ph.D Biomedical Engineering, 2004-2008 |
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Sara J. TaylorStaff Scientist, Washington University School of Medicine
Ph.D. Biomedical Engineering, 2001-2005 |
Lindsey CrawfordPost Doctoral Researcher, 2015-2016 PhD Chemical Engineering, B.S. Chemical Engineering Engineering, Research Areas: Adult stem cells, differentiation, drug delivery |
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Amy HarkinsProfessor, St. Louis University Ph.D. Neuroscience and Biophysics, M.S. Neuroscience, B.S. Biology, Research Areas: Bioactive glass Sabbatical 2011-2012 |
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Emily CrownoverPh.D. University of Washington-Seattle, B.S. University of Missouri-Columbia, Post-Doc 2010-2011 |
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Dustin J. MaxwellPh.D. University of Washington-Seattle, Post-Doct 2002-2004 |
Rachel S SomvarapuMasters/BS, Biomedical Engineering,
Research Areas: Microfluidics, biomimetics |
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Lin BaiL'oreal M.S. Biomedical Engineering, 2011-2012 |
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Stan ParkerConsultant, Accenture B.S/M.S. Biomedical Engineering, 2006-2008 |
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Allison RaderJD Saint Louis University B.S/M.S. Biomedical Engineering, 2005-2008 |
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Tracey FaxelStaff Perfusionist, Rush University B.S/M.S. Biomedical Engineering, 2005-2007 |
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Maria DoukasPhD Candidate Northwestern University B.S/M.S. Biomedical Engineering, 2003-2005 |
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Brandon HicksM.D. Candidate, University of Arkansas B.S/M.S. Biomedical Engineering, 2003-2004 |
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Edgar ScottM.S. Biomedical Engineering, 2000-2001 |
Mary Alice SalazarLab manager, Staff Scientist, 2017-2022 M.S. Chemistry COMSET Graduate Fellow Clemson UniversityB.S. Biochemistry, B.A. Biology Research Areas: Scaffold for nerve regeneration, growth factor delivery, spinal cord and peripheral nerve injury, microspheres |
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Vanessa Page BarthPh.D Candidate, 2017-2020 B.S. Biomedical Engineering, Abbott Electrophysiology, Salt Lake City, UT |
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Sara OswaldM.S. Mechanical Engineering, B.S. Mechanical Engineering, |
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Michael SaundersChemical and Biomolecular Engineering, Class of 2016 AMGEN Scholar Summer 2015 |
Alaynah MurphyBiomedical Engineering, Class of 2022 University of Texas at Austin Research Areas: Biomaterials, growth factor delivery, drug delivery, spinal cord regeneration, hydrogels |
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Miriam GonzalezBiomedical Engineering, Class of 2022 University of Texas at Austin Research Areas: Stem cell engineering, differentiation, V1 interneurons |
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Carlos RuffoBiomedical Engineering, Class of 2021 University of Texas at Austin Research Areas: Microfluidics device, interneurons |
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Shawn HuangBiomedical Engineering, Class of 2020 University of Texas at Austin Research Areas: Microfluidics device, interneurons |
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Nikita GhoshBiomedical Engineering, Class of 2020 University of Texas at Austin Research Areas: Investigating astrocyte ECM/HA-based hydrogels and their role in spinal cord regeneration |
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Manwal HarbBiomedical Engineering, Class of 2019 University of Texas at Austin Research Areas: Stem cell induction, differentiation, characterization of induced V0 interneurons |
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Oliver ZhaoBiomedical Engineering, Class of 2020 University of Texas at Austin Research Areas: Stem cell engineering and differentiation |
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James ChoiBiomedical Engineering, Class of 2020 University of Texas at Austin Research Areas: Peripheral nerve regeneration using Schwann cells and growth factors |
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Peter KennyBiomedical Engineering, Class of 2018 University of Texas at Austin Research Areas: Investigating the roles of astrocyte in spinal cord regeneration |
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Zach HartmanBiomedical Engineering, Class of 2018 University of Texas at Austin Research Areas: Charaterizing interneuron V0 |
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Logan GroneckBiomedical Engineering, Class of 2019 Washington University in St. Louis Research Areas: Growth factors, interneurons |
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Divya JoshiBiomedical Engineering, Class of 2018 Washington University in St. Louis Research Areas: Stem cell engineering |
Imani PaulBiomedical Engineering, Class of 2017 uSTAR Scholars Program Washington University in St. Louis 2014-2015 |
Mary MunsellBiomedical Engineering, Class of 2018 Summer 2015 |
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Clark IngramB.S. Biomedical Engineering, 2013-2014 |
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Kathryn MoorePhD Candidate, University of North Carolina AMGEN Scholar B.S. Biomedical Engineering, Summer 2014 |
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Robin HarlandB.S. Chemistry, 2013 |
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Cara Gonzalez WelkerPhD Candidate, Stanford University Amgen Scholar, URM B.S. Biomedical Engineering, Summer 2012, Winter 2013 |
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Jessica ButtsPhD Candidate, Gladstone Institutes B.S. Biomedical Engineering, 2011-2013 |
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Chelsea BrownMD Candidate, University of Ohio B.S. Biomedical Engineering, 2009-2013 |
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Nicole ApplebaumMcKelvey Scholar B.S. Chemical Engineering, 2011-2012 |
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Tyger HowellPhD Candidate, Northwestern University USTAR, URM B.S. Biomedical Engineering, 2010-2012 |
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Jasmine KwasaPhD Candidate, Boston University uSTAR Scholars Program B.S. Biomedical Engineering, 2009-2013 |
The need for new materials for the treatment of human disease presents many exciting research opportunities. The field of biomaterials and tissue engineering has recently experienced a paradigm shift due to a rapid increase in the understanding of the biological mechanisms underlying many diseases. My research interests focus on cellular and molecular aspects of biomaterials and tissue engineering. This research is highly interdisciplinary, combining an understand of biology, chemistry, and biomedical engineering to develop new bioactive materials, which can enhance tissue regeneration and cell survival after transplantation. The bioactive signals that will be provided by integrated tissue engineering scaffolds include signals for cell-type specific adhesion and migration, as well as growth factors to promote cell proliferation and differentiation. |
NPC transplanted 2 weeks after spinal cord injury survival (green cells)and form rosette-like structures at 1 week. Cells near the outside of the rosettes express beta-tubulin III, a marker of neuronal differentiation (red). |
The overall goal of this project is to use novel biomaterials to allow controlled release of growth factors from scaffolds that facilitates regeneration in the adult mammalian spinal cord after injury. These “tissue engineering” scaffolds will provide two critical mechanisms for enhancing spinal cord regeneration: 1) they will provide a permissive scaffold for cellular migration and axonal outgrowth of host and/or transplanted cells into and across the lesion site (thus reducing the inhibitory environment normally found in spinal cord lesions), and 2) they will serve as a drug delivery vehicle for the controlled release of one or more neurotrophic factors to promote axonal regrowth, cellsurvival, and differentiation of transplanted neural progenitor cells after spinal cord injury. The scaffolds are drug-delivery systems consisting of fibrin matrices containing growth factors that are released in a sustained manner during tissue regeneration. By providing both a permissive matrix to serve as a substrate for axonal regeneration and soluble stimuli, in the form of neurotrophic factors, to enhance fiber sprouting, both the extracellular and the cellular environment within the spinal cord will be dramatically altered thereby enhancing the potential for regeneration within the central nervous system (CNS). These scaffolds can be further modified through the addition of embryonic stem (ES) cell derived neural progenitor cells (NPCs) during polymerization. The NPCs can serve as a source of neurotrophic factors and/or a source of cells to replace those lost due to injury. |
Embryonic stem cells induced to form progenitor motor neurons. The resulting population is heterogeneous and includes A) neural progenitor cells (red), B) motor neuron progenitor (red) , C) undifferentiated stem cells (red), and D) neurons (green). All cell nuclei are labeled with Hoechst (blue) |
Embryonic stem cells provide a potential source for cell replacement following injury. In the case of spinal cord injury, stem cells can be utilized to repopulate the injured cord with neurons and glial cells that otherwise have limited self-renewal. To obtain neural cells (neurons and glial cells), stem cells are first induced to form neural progenitor cells that can differentiate following transplantation into the injured cord. The induction and subsequent differentiation, however, leads to a heterogeneous population of cells that can be detrimental to repair. Recent studies in my lab have shown that undifferentiated stem cells remaining after the induction can form tumors following transplantation and present significant safety concerns. Furthermore, stem cells that were successfully induced into neural progenitor cells may differentiate into neurons, oligodendrocytes, or astrocytes, each with varying effects on spinal cord regeneration. To account for this variability, our lab is investigating methods for isolating purified cell populations for transplantation including: 1) adding additional developmental signals during the induction process to further restrict the population of progenitor cells obtained, 2) removing undifferentiated stem cells after the induction, 3) using the fibrin matrices with incorporated growth factors to improve the survival of transplanted progenitor cells and promote differentiation into desired cell types. By transplanting select populations of cells, we hope to understand the role of each cell type in regeneration and reduce the risks often associated with embryonic stem cells. |
Growth factors are potent protein drugs that are powerful regulators of biological function. Their presence in tissues is highly regulated in both time and space. The ability to tightly regulate the release of growth factors is essential in the development of tissue engineering scaffolds. My laboratory is using combinatorial methods to design novel materials for affinity-based protein delivery. The release of proteins from affinity-base delivery systems can be optimized by changing the number of protein-binding sites in the material or by changing the affinity of the interaction between the protein and the material. The libraries of compounds developed in this project can provide a new method for the regulation of drug release profiles - regulation of the affinity of the delivery vehicle for the drug. Based on an understanding of the time course of key events required for tissue regeneration, these affinity-based protein delivery vehicles can be incorporated into tissue engineering scaffolds to provide the signals necessary to stimulate tissue regeneration on a relevant time scale. |
Our lab also develops scaffolds for drug delivery and cell transplantation for the treatment of peripheral nerve injury. We have examined the effect of growth factor delivery from biomaterial scaffolds that serve as filler for nerve guidance conduits, as a potential alternative to nerve autografts for long gap injuries. We have demonstrated that delivery of glial dervied neurotrophic factor (GDNF) and nerve growth factor (NGF) from fibrin scaffolds enhance functional regeneration compared to empty conduits in a 13 mm sciatic nerve injury model. Currently we are assessing whether these delivery systems can also be used in combination with acellular nerve allografts that have recently come on the market for clinical use. We are also studying the role of Schwann cell (SC) phenotype on motor (nerve) specific regeneration. We hypothesis that SC provide cues that help to direct motor specific regeneration, but that they may lose expression of key signals duing expansion in cell culture (typically performed prior to transplantation. We are evaluating environmental cues that are important for maintianing/restoring SC phenotype and the effect of phenotype of nerve regeneration through acellular allografts. |
McCreedy DA, Jalufka FL, Platt ME, Min SW, Kirchhoff MA, Pritchard AL, Reid SK, Manlapaz R, Mihaly E, Butts JC, Iyer NR, Sakiyama-Elbert S, Crone SA, McDevitt TC. Passive Clearing and 3D Lightsheet Imaging of the Intact and Injured Spinal Cord in Mice. Frontiers in Cellular Neuroscience. 15: 684792. PMID 34408627 DOI: 10.3389/fncel.2021.684792
Pardieck J, Harb M, Sakiyama-Elbert S. Induction of Ventral Spinal V0 Interneurons from Mouse Embryonic Stem Cells. Stem Cells and Development. PMID 34139881 DOI: 10.1089/scd.2021.0003
Stevens KR, Masters KS, Imoukhuede PI, Haynes KA, Setton LA, Cosgriff-Hernandez E, Lediju Bell MA, Rangamani P, Sakiyama-Elbert S, Finley SD, Willits RK, Koppes AN, Chesler NC, Christman KL, Allen JB, et al. Fund Black scientists. Cell. PMID 33503447 DOI: 10.1016/j.cell.2021.01.011
Shen H, Yoneda S, Sakiyama-Elbert S, Zhang Q, Thomopoulos S, Gelberman RH. Flexor Tendon Injury and Repair: The Influence of Synovial Environment on the Early Healing Response in a Canine Mode. The Journal of Bone and Joint Surgery. American Volume. PMID 33475308 DOI: 10.2106/JBJS.20.01253
Butts JC, Iyer N, White N, Thompson R, Sakiyama-Elbert S, McDevitt TC. Author Correction: V2a interneuron differentiation from mouse and human pluripotent stem cells. Nature Protocols. PMID 31705126 DOI: 10.1038/S41596-019-0266-Z
Butts JC, Iyer N, White N, Thompson R, Sakiyama-Elbert S, McDevitt TC. V2a interneuron differentiation from mouse and human pluripotent stem cells. Nature Protocols. PMID 31628445 DOI: 10.1038/S41596-019-0203-1
Wilems T, Vardhan S, Wu S, Sakiyama-Elbert S. The influence of microenvironment and extracellular matrix molecules in driving neural stem cell fate within biomaterials. Brain Research Bulletin. PMID 30898579 DOI: 10.1016/J.Brainresbull.2019.03.004
Thompson RE, Pardieck J, Smith L, Kenny P, Crawford L, Shoichet, M, Sakiyama-Elbert S. Effect of Hyaluronic Acid Hydrogels Containing Astrocyte-Derived Extracellular Matrix and/or V2a Interneurons on Histologic Outcomes following Spinal Cord Injury. Biomaterials 162, 208-223, 2018.
Pardieck J and Sakiyama-Elbert SE. Genome Engineering for Central Nervous System Injury and Disease. Current Opinion in Biotechnology 52, 89-94, 2018.
Thompson RE, Sakiyama-Elbert S. Using Biomaterials to Promote Pro-Regenerative Glial Phenotypes After Nervous System Injuries. Biomed Mater. 13(2) 024104, 2018.
Zholudeva LV, Iyer NR, Qiang L, Spruance VM, Randelman ML, White NW, Bezdudnaya T, Fischer I, Sakiyama-Elbert SE, Lane MA. Transplantation of Neural Progenitors and V2a Interneurons after Spinal Cord Injury. J Neurotrauma (in press), 2018.
Zholudeva LV, Qiang L, Marchenko V, Dougherty KJ, Sakiyama-Elbert SE, Lane MA. The Neuroplastic and Therapeutic Potential of Spinal Interneurons in the Injured Spinal Cord. Trends Neurosci. 2018.
Shen H, Jayaram R, Yoneda S, Linderman SW, Sakiyama-Elbert S, Xia Y, Gelberman RH, Thomopoulos S. The effect of adipose-derived stem cell sheets and CTGF on early flexor tendon healing in a canine model. Scientific Reports, In press, 2018.
Linderman SW, Shen H, Yoneda S, Jayaram R, Tanes, ML, Sakiyama-Elbert SE, Xia Y, Thomopoulos S, Gelberman RH. Effect of connective tissue growth factor delivered via porous sutures on the proliferative stage of intrasynovial tendon repair. Journal of Orthopaedic Research 36(7): 2052-2063, 2018.
Thompson RE, Lake A, Kenny P, Saunders M, Sakers K, Iyer N, Dougherty JD, Sakiyama-Elbert SE. Different Mixed Astrocyte Populations Derived from Embryonic Stem Cells have Variable Neuronal Growth Support Capacities. Stem Cells Dev. 26(22): 1597-1611, 2017.
Iyer NR, Wilems TS, Sakiyama-Elbert SE. Stem cells for spinal cord injury: Strategies to inform differentiation and transplantation. Biotechnol Bioeng. 114(2):245-259, 2017.
Walter C, Crawford L, Lai M, Toonen JA, Pan Y, Sakiyama-Elbert S, Gutmann DH, Pathak A. Increased Tissue Stiffness in Tumors from Mice with Neurofibromatosis-1 Optic Glioma. Biophysical Journal 112(8):1535-1538, 2017.
Gelberman RH, Linderman SW, Jayarm R, Dikina A,
Ee X, Yan Y, Hunter DA, Schellhardt L, Sakiyama-Elbert SE, Mackinnon SE, Wood MD. Transgenic SCs expressing GDNF-IRES-DsRed impair nerve regeneration within acellular nerve allografts. Biotechnol Bioeng 114(9):2121-2130, 2017.
Iyer N, Huettner JE, Butts JC, Brown CR and Sakiyama-Elbert SE. Generation of Highly Enriched V2a Interneurons from Mouse Embryonic Stem Cells. Experimental Neurology, 277:305-16, 2016.
Shen H, Kormpakis I, Havlioglu N, Linderman SW, Sakiyama-Elbert S, Erickson IE, Zarembinsk T, Silva MJ, Gelberman RH, Thomopoulos S. The effect of mesenchymal stromal cell sheets on the inflammatory stage of flexor tendon healing. Stem Cell Res Therapy, 7:144, 2016.
Sand JP, Park AM, Bhatt N, Desai SC, Marquardt L, Sakiyama-Elbert SE, Paniello RC. A Comparison of Conventional, Revascularized and Bioengineered Methods of Recurrent Laryngeal Nerve Reconstruction. JAMA Otolaryngology-Head & Neck Surgery, 142(6):526-532, 2016.
Gelberman RH, Shen H, Kormpakis I, Rothrauff B, Yang G, Tuan RS, Xia Y, Sakiyama-Elbert S, Silva MJ, Thomopoulos S. The effect of adipose-derived stromal cells and BMP12 on intrasynovial tendon repair: A biomechanical, biochemical, and proteomics study. J Orthop Res, 34(4):630-40, 2016.
Xu H, Iyer N, Huettner JE, and Sakiyama-Elbert SE . A puromycin selectable cell line for the enrichment of mouse embryonic stem cell derived V3 interneurons. Stem Cell Research & Therapy , 6:220, 2015. (Open Access)
Marquardt LM, Ee X, Iyer N, Hunter D, Mackinnon SE, Wood M, and Sakiyama-Elbert SE . Finely Tuned Temporal and Spatial Delivery of GDNF Promotes Enhanced Nerve Regeneration in a Long Nerve Defect Model. Tissue Engineering Part A , 21:2852-2864, 2015.
Wilems TS, Pardieck J, Iyer N, Sakiyama-Elbert SE . Combination Therapy of Stem Cell Derived Neural Progenitors and Drug Delivery of Anti-Inhibitory Molecules for Spinal Cord Injury. Acta Biomaterialia , 28:23-32, 2015.
Xu H, Sakiyama-Elbert SE . Directed Differentiation of V3 Interneurons from Mouse Embryonic Stem Cells. Stem Cells and Development , 24:2723-2732, 2015.
Wilems TS, Sakiyama-Elbert SE . Sustained dual drug delivery of anti-inhibitory molecules for treatment of spinal cord injury. J Controlled Release, 213:103-111, 2015.
Hoben G, Yan Y, Iyer N, Newton P, Hunter DA, Moore AM, Sakiyama-Elbert SE., Wood, MD and Mackinnon SE. Comparison of acceular nerve allograft modification with Schwann cells or VEGF. Hand, 10(3):396-402, 2015.
Marquardt LM, Sakiyama-Elbert SE. GDNF preconditioning can overcome Schwann cell phenotypic memory. Experimental Neurology, 265:1-7, 2015.
Manning CN, Martel C, Sakiyama-Elbert SE, Silva MJ, Shah S, Gelberman RH Thomopoulos. Adipose-derived mesenchymal stromal cells modulate tendon fibroblast responses to macrophage-induced inflammation in vitro. Stem Cell Research and Therapy., 6(74), 2015.
McCreedy D, Wilems T, Xu H, Butts J, Brown C, Smith A, Sakiyama-Elbert, SE. Survival, Differentiation, and Migration of High-Purity Mouse Embryonic Stem Cell-derived Progenitor Motor Neurons in Fibrin Scaffolds after Sub-Acute Spinal Cord Injury. Biomaterials Science, 2: 1672-1682, 2014.[Pubmed Central]
McCreedy DA, Brown CR, Butts JC, Xu H, Huettner J, Sakiyama-Elbert, SE. A New Method for Generating High Purity Motoneurons From Mouse Embryonic Stem Cells. Biotechnology and Bioengineering, 111:2041-2055, 2014.[Pubmed Central]
Brown CR, Butts JC, McCreedy DA, and Sakiyama-Elbert, SE. Generation of V2a interneurons from mouse embryonic stem cells. Stem Cells and Development, 23:1765-1776, 2014.[Pubmed Central]
Jesuraj NJ, Marquardt LM, Kwasa J,Sakiyama-Elbert SE. Glial cell line-derived neurotrophic factor promotes increased phenotypic marker expression in femoral sensory and motor-derived Schwann cell cultures. Experimental Neurology, 257:10-18, 2014.[Pubmed Central]
Wu-Fienberg Y, Marquardt L,Newton P, Johnson PJ, Mackinnon SE, Sakiyama-Elbert SE, Moore AM, Wood MD. Viral transduction of primary Schwann cells using a Cre-lox system to regulate GDNF expression. Biotechnology and Bioengineering , 111(9):1886-94, 2014.
Marquardt LM, Day D, Sakiyama-Elbert SE, and Harkins AB. Effect of Borate Based Bioactive Glass on Neuron viability and Neurite Extension. Journal of Biomedical Materials Research, 102(8):2767-75, 2014.
Jesuraj NJ, Santosa KB, MacEwan MR, Moore AM, Ksukurthi R, Ray WZ Flagg ER, Hunter DA, Borshcel GH, Johnson PJ, Mackinnon SE, Sakiyama-Elbert SE. Schwann cells seeded in acellular nerve grafts improve functional recovery. Muscle and Nerve, 49(2):267-76, 2014.[Pubmed Central]
Lu X, Kim-Han JS,Harmon, S,Sakiyama-Elbert SE, O'Malley KL,The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons. Molecular Neurodegeneration , 9:17,2014.
Manning CN, Havlioglu N, Knutsen E,Sakiyama-Elbert SE, Silva MJ, Thomopoulos S, Gelberman RH.The early inflammatory response after flexor tendon healing: A gene expression and histological analysis. J Orthop Res., 32(5):645-52, 2014.
Sakiyama-Elbert, SE. Incorporation of Heparin into Biomaterials. Acta Biomaterialia, 10(4):1581-1587, 2014.
Shen H, Gelberman RH, Silva MJ, Sakiyama-Elbert SE, Thomopoulos S. BMP 12 induces tenogenic differentiation of adipose-derived stem cells. PLoS ONE,, 8(10):e77613,2013.
Manning CN, Schwartz AG, Liu W, Xie J, Havlioglu N, Sakiyama-Elbert SE, Silva MJ, Xia Y, Gelberman RH, Thomopoulos S. Controlled delivery of mesenchymal stem cells and growth factors using a nanofiber scaffold for tendon repair. Acta Biomaterialia, 9: 6905-14,2013.
Marquardt LM, Sakiyama-Elbert SE. Engineering Peripheral Nerve Repair. Current Opinion in Biotechnology, 24: 887-892, 2013.
McCreedy,DA, Silverman C, Gottleib DI, and Sakiyama-Elbert, SE. Combination Therapies in the CNS: Engineering the Environment. Neuroscience Letters , 519: 115-121 2012. [Pubmed Central]
McCreedy,DA, Silverman C, Gottleib DI, and Sakiyama-Elbert, SE. Transgenic Enrichment of Mouse Embryonic Stem Cell-derived Progenitor Motor Neurons. Stem Cell Research , 8: 368-378, 2012. [Pubmed Central]
Jesuraj NJ, Nguyen P, Wood MD, Moore AM, Mackinnon SE, Borschel GH, Sakiyama-Elbert SE. Differential Gene Expression in Motor and Sensory Schwann Cells in the Rat Femoral Nerve. Journal of Neuroscience Research, 90(1):96-104, 2012. [Pubmed Central]
Lu X, Kim-Han JS, O';Malley KL, Sakiyama-Elbert SE. A Microdevice Platform for Visualizing Mitochondrial Tansport in Aligned Dopaminergic Axons. J Neuroscience Methods, 209(1):35-39, 2012.
Moore NM and Sakiyama-Elbert SE. Analysis of Cell Binding and Internalization of Multivalent PEG-Based Gene Delivery Vehicles. IEEE Transactions on Nanobioscience, 11(1): 54-61, 2012.
Sakiyama-Elbert SE, Johnson PJ, Hodgetts SI, Plant GW, Harvey AR. Scaffolds to promote spinal cord regeneration. Handbook of Clinical Neurology, 109:575-94, 2012.
Jesuraj NJ, Santosa KB, Newton P, Liu Z, Hunter DA, Mackinnon SE, Sakiyama-Elbert SE, Johnson PJ. Systematic Evaluation of Schwann Cell Injection into Acellular Cold-Preserved Nerve Grafts. Journal of Neuroscience Methods, 197(2):209-215, 2011. [Pubmed Central]
Wolfe, C, Kim, HM, Sakiyama-Elbert S, Galatz LM, Havlioglu N, Thomopoulos S. Sustained delivery of transforming growth factor beta three enhnaces tendon-to-bone healing in a rat model. Journal of Orthopaedic Research,29(7):1099-105, 2011.
Johnson, PJ, Tatara, A, McCreedy, DA, Shiu A, Sakiyama-Elbert, SE. Tissue-engineered fibrin scaffolds containing neural progenitors enhance functional recovery in a subacute model of SCI. Soft Matter , 6: 5127-5137, 2010. [Pubmed Central]
Johnson PJ, Tatara A, Shiu A, Sakiyama-Elbert, SE. Controlled release of neurotrophin-3 and platelet-derived growth factor from fibrin scaffolds containing neural progenitor cells enhances survival and differentiation into neurons in a subacute model of SCI. Cell Transplantation , 19: 89-101, 2010. [Pubmed Central]
Johnson, PJ, Parker, SR, Sakiyama-Elbert, SE. Fibrin-based tissue engineering scaffolds enhance neural fiber sprouting and delays the accumulation of reactive astrocytes at the lesion in a subacute model of spinal cord injury. Journal of Biomedical Materials Research , 92(1):152-63, 2010. [Pubmed Central]
Moore AM, Wood MD, Chenard K, Hunter DA, Mackinnon SE, Sakiyama-Elbert SE, Borschel GH. Controlled Delivery of Glial Cell Line-Derived Neurotrophic Factor Enhances Motor Nerve Regeneration. Journal of Hand Surgery, 35(12):2008-17,2010.
Wood, MD, MacEwan MR, French AR, Moore, AM, Hunter, Mackinnon, SE, Moran DW, Borschel, GH, and Sakiyama-Elbert, SE. Fibrin Matrices with Affinity-based Delivery Systems and Neurotrophic Factors Promote Functional Nerve Regeneration. Biotechnology and Bioengineering, 106:970-9, 2010.
Wood, MD, Moore, AM, Hunter, Mackinnon, SE, and Sakiyama-Elbert, SE. Heparin-Binding-Affinity-Based Delivery Systems Releasing Nerve Growth Factor Enhance Sciatic Nerve Regeneration. Journal of Biomaterials Science Polymer Edition, 21:771-87, 2010.
Thomopoulos S, Kim HM, Das R, Silva MJ, Sakiyama-Elbert S, Amiel D, Gelberman RH. The effects of exogenous basic fibroblast growth factor on intrasynovial flexor tendon healing in a canine model. Journal of Bone and Joint Surgery American, 92:2285-93, 2010. [Pubmed Central]
Thomopoulos S, Das R, Sakiyama-Elbert S, Silva MJ, Charlton N, Gelberman RH. bFGF and PDGF-BB for tendon repair: controlled release and biologic activity by tendon fibroblasts in vitro. Annals of Biomedical Engineering, 38:225-34, 2010. [Pubmed Central]
Johnson, PJ, Parker, SR, Sakiyama-Elbert, SE. Controlled release of neurotrophin-3 from fibrin-based tissue engineering scaffolds enhances neural fiber sprouting following subacute spinal cord injury. Biotechnology and Bioengineering , 104(6):1207-14, 2009. [Pubmed Central]
Willerth, SM, and Sakiyama-Elbert, SE. Kinetic Analysis of Neurotrophin-3 Mediated Differentiation of Embryonic Stem Cells into Neurons. Tissue Engineering , 15:307-18, 2009. [Pubmed Central]
Wood, MD, Moore, AM, Hunter, DA, Tuffaha, S, Borschel, GH, Mackinnon, SE, and Sakiyama-Elbert, SE. Affinity-based Release of Glial-Derived Neurotrophic Factor from Fibrin Matrices Enhances Sciatic Nerve Regeneration. Acta Biomaterialia, 5:959-68, 2009.[Pubmed Central]
Moore, NM, Sheppard, CL, Sakiyama-Elbert, SE. Characterization of a Multifunctional PEG-Based Gene Delivery System Containing Nuclear Localization Signals and Endosomal Escape Peptides. Acta Biomaterialia 5:854-64., 2009.
Thomopoulos S, Das R, Silva MJ, Sakiyama-Elbert S, Harwood FL, Zampiakis E, Kim HM, Amiel D, Gelberman RH. Enhanced flexor tendon healing through sustained delivery of PDGF-BB. Journal of Orthopaedic Research, 27:1209-15, 2009. [Pubmed Central]
Xie J, Macewan MR, Willerth SM, Li X, Moran DW, Sakiyama-Elbert SE, Xia Y. Conductive Core-Sheath Nanofibers and Their Potential Application in Neural Tissue Engineering. Advanced Functional Materials, 19:2312-2318, 2009.
Xie J, MacEwan MR, Li X, Sakiyama-Elbert SE, Xia Y. Neurite Outgrowth on Nanofiber Scaffolds with Different Orders, Structures, and Surface Properties. ACS Nano, 3 (5), 1151–1159, 2009.[Pubmed Central]
Xie, J, Willerth, SM, LI, X Rader, A, MacEwan, MR, Gottlieb, DI, Sakiyama-Elbert, SE, and Xia, Y. The Differentiation of Embryonic Stem Cells Seeded on Electrospun Nanofibers into Neural Lineages. Biomaterials, 30: 354-62, 2009.[Pubmed Central]
Willerth, SM, Rader; A, Sakiyama-Elbert, SE. The Effect of Controlled Growth Factor Delivery on Embryonic Stem Cell Differentiation Inside of Fibrin Scaffolds. Stem Cell Research , 1: 205-218, 2008. [Pubmed Central]
Wood MD, Borschel GH, and Sakiyama-Elbert SE. Controlled release of glial-derived neurotrophic factor from fibrin matrices containing an affinity-based delivery system. Journal of Biomedical Materials Research Part A, 89:909-918, 2008.
Wood, MD and Sakiyama-Elbert, SE. Release Rate Controls Biological Activity of Nerve Growth Factor Released from Fibrin Matrices Containing Affinity-Based Delivery Systems. Journal of Biomedical Materials Research Part A, 84:300-312, 2008.
Moore, NM, Sheppard, CL, Barbour, TR, Sakiyama-Elbert, SE. The Effect of Endosomal Escape Peptides on In Vitro Gene Delivery of Polyethylene Glycol - Based Vehicles. Journal of Gene Medicine,10: 1134-1149, 2008.
Moore, NM, Barbour, TR, Sakiyama-Elbert, SE. Synthesis and Characterization of Four-Arm Poly (ethylene glycol) Based Gene Delivery Vehicles Coupled to Integrin and DNA Binding Peptides. Molecular Pharmaceutics, 5:140-150, 2008.
Sakiyama-Elbert S, Das R, Gelberman RH, Harwood F, Amiel D, Thomopoulos S. Controlled release kinetics and biologic activity of PDGF-BB for use in flexor tendon repair. Journal of Hand Surgery[American], 33:1548-57, 2008.[Pubmed Central]
Willerth, SM, and Sakiyama-Elbert, SE, Cell Therapy for Spinal Cord Regeneration. Advanced Drug Delivery Reviews, 60:263-276, 2008.
Willerth SM, Sakiyama-Elbert SE. Combining stem cells and biomaterial scaffold for constructing tissues and cell delivery. StemBook, Cambridge (MA): Harvard Stem Cell Institute: 2008.
Willerth, SM, Faxel, TE, Gottlieb, D, and Sakiyama-Elbert, SE. The Effects of Soluble Growth Factors on Embryonic Stem Cell Differentiation Inside of Fibrin Scaffolds. Stem Cells , 25(9):2235-2244, 2007. [Pubmed Central]
Willerth, SJ, Johnson, PJ, Maxwell, DJ, Parsons, SP, Sakiyama-Elbert, SE. Rationally Designed Peptides for Controlled Release of Nerve Growth Factor from Fibrin Matrices Journal of Biomedical Materials Research Part A , 80A(1):13-23, 2007.
Schmieder, AH, Grabski, LE, Moore, NM, Dempsey, LA, Sakiyama-Elbert, SE. Development of Novel Poly(ethylene glycol) Based Vehicles for Gene Delivery. Biotechnology and Bioengineering , 96: 967-976, 2007.
Thomopoulos, S, Zaegel, M, Das, R, Harwood FL, Silva, MJ, Amiel, D, Sakiyama-Elbert,S , Gelberman, RH. A novel method for growth factor delivery in tendon repair: Enhanced healing through sustained delivery of PDGF-BB. Journal of Orthopaedic Research, 25(10): 1358-1368, 2007.
Gelberman, RH, Thomopoulos, S, Sakiyama-Elbert, S, Das, R, Silva, M. The Early Effects of Sustained Platelet Derived Growth Factor Administration on the Functional and Structural Properties of Repaired Intrasynovial Flexor Tendons The Journal of Hand Surgery, 32(3): 373-379, 2007.
Willerth, SM, and Sakiyama-Elbert, SE, Approaches to neural tissue engineering using scaffolds for drug delivery. Advanced Drug Delivery Reviews,., 59:325-338, 2007[Pubmed Central]
Taylor, SJ and Sakiyama-Elbert, SE. Effect of Controlled Delivery of Neurotrophin-3 from Fibrin on Spinal Cord Injury in a Long Term Model. Journal of Controlled Release , 116:204-210, 2006. [Pubmed Central]
Willerth, SM, Arendas KJ, Gottlieb, DI, Sakiyama-Elbert, SE. Optimization of Fibrin Scaffolds for Differentiation of Murine Embryonic Stem Cells into Neural Lineage Cells. Biomaterials , 27(36):5990-6003, 2006. [Pubmed Central]
Taylor, SJ Rosenzweig, ES, McDonald, JW, Sakiyama-Elbert, SE. Controlled Delivery of Neurotrophin-3 from Fibrin Scaffolds Enhances Neural Fiber Sprouting After Spinal Cord Injury Model. Journal of Controlled Release, 113:225-235, 2006 . [Pubmed Central]
Maxwell, DJ, Hicks, BC, Parson, S, Sakiyama-Elbert, SE. Development of Rationally Designed Affinity-based Drug Delivery Systems, Acta Biomaterialia , 1:101-113, 2005.
Taylor, S.J., McDonald, J.W., and Sakiyama-Elbert, SE. Controlled release of neurotrophin-3 from fibrin gels for spinal cord injury. J Controlled Release, 98, 281-294, 2004.
Lee AC, Yu VM, Lowe JB, Brenner MJ, Hunter DA Mackinnon SE, Sakiyama-Elbert SE.Controlled release of nerve growth factor enhances sciatic nerve regeneration. Experimental Neurology, 84: 295-303, 2003.
Sakiyama-Elbert, S.E., Panitch, A and Hubbell, J.A., Development of growth factor fusion proteins for cell-triggered drug delivery, FASEB Journal, 15: 1300-1302, 2001.
Zisch, A.H., Schenk, U., Schense, J.C., Sakiyama-Elbert, S.E., and Hubbell, J.A., Covalently conjugated VEGF-fibrin matrices for endothelialization, J. Controlled Release,, 72: 101-113, 2001.
Sakiyama, S.E. and Hubbell, J.A., FUNCTIONAL BIOMATERIALS: Design of Novel Biomaterials, Annual Review of Materials Research, 31: 183-201, 2001.
Sakiyama-Elbert, S.E. and Hubbell, J.A., Controlled release of nerve growth factor from a heparin-containing fibrin-based cell ingrowth matrix, J. Controlled Release,, 69: 149-158, 2000.
Sakiyama-Elbert, S.E. and Hubbell, J.A., Development of Fibrin Derivatives for Controlled Release of Heparin-Binding Growth Factors J. Controlled Release, 65: 389-402, 2000.
Sakiyama, S.E. and Hubbell, J.A., Heparin-binding Peptides Enhance Neurite Extension in Three Dimensional Fibrin Gels, FASEB Journal, 13: 2214-2224, 1999.
Sakiyama-Elbert SE, Johnson PJ, Hodgetts SI, Plant GW, Harvey AR. Scaffolds to promote spinal cord regeneration. Handbook of Clinical Neurology, 109:575-94, 2012.
Willerth SM, Sakiyama-Elbert SE. Combining stem cells and biomaterial scaffold for constructing tissues and cell delivery. StemBook, Cambridge (MA): Harvard Stem Cell Institute: 2008.
The University of Texas at Austin
Biomedical Engineering Building Room 4.110
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Austin, TX 78712
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Email: sakiyama@utexas.edu